AK3 Mouse Monoclonal Antibody [Clone ID: OTI3G1]

CAT#: CF501834

Carrier-free (BSA/glycerol-free) AK3 mouse monoclonal antibody, clone OTI3G1 (formerly 3G1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AK3 (RC204408, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AK3. Positive lysates LY414079 (100ug) and LC414079 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (TA501834)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AK3 mouse monoclonal antibody. (TA501834)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AK3 mouse monoclonal antibody. (TA501834)

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  Anti-AK3 mouse monoclonal antibody (TA501834) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AK3 (RC204408).

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  HEK293T cells transfected with either RC204408 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-AK3 antibody (TA501834), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3G1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human AK3 (NP_057336) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.4 kDa
• Gene Name: Homo sapiens adenylate kinase 3 (AK3), transcript variant 1, mRNA.
• Database Link:
  NP_057366
  Entrez Gene 26956 RatEntrez Gene 56248 MouseEntrez Gene 50808 Human
  UniProt ID Q9UIJ7
• Synonyms: AK3L1; AK6; AKL3L; AKL3L1; FIX

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pyrimidine metabolism