MICAL1 Mouse Monoclonal Antibody [Clone ID: OTI6H4]

CAT#: CF501842

Carrier-free (BSA/glycerol-free) MICAL1 mouse monoclonal antibody, clone OTI6H4 (formerly 6H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MICAL1 (RC208308, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MICAL1. Positive lysates LY402940 (100ug) and LC402940 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MICAL1 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501842)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501842)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MICAL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501842)

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  Anti-MICAL1 mouse monoclonal antibody (TA501842) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MICAL1 (RC208308).

• HEK293T cells transfected with either RC208308 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MICAL1 antibody (TA501842), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-MICAL1 antibody (TA501842), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI6H4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MICAL1 (NP_073602) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 117.7 kDa
• Gene Name: Homo sapiens microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant 1, mRNA.
• Database Link:
  NP_073602
  Entrez Gene 64780 Human
  UniProt ID Q8TDZ2
• Synonyms: MICAL; MICAL-1; NICAL