ALDH1L1 Mouse Monoclonal Antibody [Clone ID: OTI5G8]

CAT#: CF501870

Carrier-free (BSA/glycerol-free) ALDH1L1 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)

Product Images

Anti-ALDH1L1 mouse monoclonal antibody (TA501870) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1 (RC213720).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ALDH1L1 (RC213720, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ALDH1L1. Positive lysates LY415919 (100ug) and LC415919 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ALDH1L1 monoclonal antibody.

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  HEK293T cells transfected with either RC213720 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ALDH1L1 antibody (TA501870), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-ALDH1L1 antibody (TA501870), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ALDH1L1 mouse monoclonal antibody. (TA501870)

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Specifications

Product Data

• Clone Name: OTI5G8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ALDH1L1 (NP_036322) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 98.6 kDa
• Gene Name: Homo sapiens aldehyde dehydrogenase 1 family member L1 (ALDH1L1), transcript variant 2, mRNA.
• Database Link:
  NP_036322
  Entrez Gene 64392 RatEntrez Gene 100855730 DogEntrez Gene 10840 Human
  UniProt ID O75891
• Background: The protein encoded by this gene catalyzes the conversion of 10-formyltetrahydrofolate, NADP, and water to tetrahydrofolate, NADPH, and carbon dioxide. The encoded protein belongs to the aldehyde dehydrogenase family and is responsible for formate oxidation in vivo. Deficiencies in this gene can result in an accumulation of formate and subsequent methanol poisoning. [provided by RefSeq]
• Synonyms: 10-fTHF; 10-FTHFDH; FDH; FTHFD

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: One carbon pool by folate