Ribonuclease Inhibitor (RNH1) Mouse Monoclonal Antibody [Clone ID: OTI4G4]

CAT#: CF501874

Carrier-free (BSA/glycerol-free) RNH1 mouse monoclonal antibody, clone OTI4G4 (formerly 4G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNH1 (RC208360, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNH1. Positive lysates LY401028 (100ug) and LC401028 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RNH1 mouse monoclonal antibody. (TA501874)

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  Anti-RNH1 mouse monoclonal antibody (TA501874) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNH1 (RC208360).

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  HEK293T cells transfected with either RC208360 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RNH1 antibody (TA501874), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RNH1 antibody (TA501874), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4G4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100, IHC: 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RNH1 (NP_002930) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.8 kDa
• Gene Name: Homo sapiens ribonuclease/angiogenin inhibitor 1 (RNH1), transcript variant 1, mRNA.
• Database Link:
  NP_002930
  Entrez Gene 6050 Human
  UniProt ID P13489
• Background: Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo. [supplied by OMIM]
• Synonyms: RAI; RNH