DUSP27 (DUPD1) Mouse Monoclonal Antibody [Clone ID: OTI6C6]

CAT#: CF501880

Carrier-free (BSA/glycerol-free) DUPD1 mouse monoclonal antibody, clone OTI6C6 (formerly 6C6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUPD1 (RC214361, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUPD1. Positive lysates LY424030 (100ug) and LC424030 (20ug) can be purchased separately from OriGene.

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  Anti-DUPD1 mouse monoclonal antibody (TA501880) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DUPD1 (RC214361).

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  HEK293T cells transfected with either RC214361 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DUPD1 antibody (TA501880), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6C6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DUPD1(NP_001003892) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 25.2 kDa
• Gene Name: Homo sapiens dual specificity phosphatase 29 (DUSP29), transcript variant 1, mRNA.
• Database Link:
  NP_001003892
  Entrez Gene 338599 Human
  UniProt ID Q68J44
• Synonyms: DUSP27; FMDSP

Reference Data

• Protein Families: Phosphatase