Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10]

CAT#: CF501907

Carrier-free (BSA/glycerol-free) GAS7 mouse monoclonal antibody, clone OTI8H10 (formerly 8H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates LY418527 (100ug) and LC418527 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (TA501907). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-GAS7 mouse monoclonal antibody (TA501907) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7 (RC215256).

• HEK293T cells transfected with either RC215256 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GAS7 antibody (TA501907), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-GAS7 antibody (TA501907), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-GAS7 antibody (TA501907), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI8H10
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:500-1:2000, IF: 1:100, FLOW: 1:100, IHC: 1:50-1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.0 kDa
• Gene Name: growth arrest specific 7
• Database Link:
  NP_003635
  Entrez Gene 14457 MouseEntrez Gene 85246 RatEntrez Gene 8522 Human
  UniProt ID O60861
• Background: Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq]
• Synonyms: GAS7; MLL

Reference Data

• Protein Families: Transcription Factors