Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2G3]

CAT#: CF501935

Carrier-free (BSA/glycerol-free) GSS mouse monoclonal antibody, clone OTI2G3 (formerly 2G3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSS (RC203174, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSS. Positive lysates LY424876 (100ug) and LC424876 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSS monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-GSS monoclonal antibody (1:200).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GSS-Knockout 293T cells (KO, Cat# LC811047) were separated by SDS-PAGE and immunoblotted with anti-GSS monoclonal antibody TA501935, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  HEK293T cells transfected with either RC203174 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSS antibody (TA501935), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2G3
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.2 kDa
• Gene Name: Homo sapiens glutathione synthetase (GSS), transcript variant 1, mRNA.
• Database Link:
  NP_000169
  Entrez Gene 14854 MouseEntrez Gene 25458 RatEntrez Gene 442962 DogEntrez Gene 2937 Human
  UniProt ID P48637
• Background: Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency. [provided by RefSeq]
• Synonyms: GSHS; HEL-S-64p; HEL-S-88n

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glutathione metabolism, Metabolic pathways