OTUB2 Mouse Monoclonal Antibody [Clone ID: OTI11B3]

CAT#: CF501946

Carrier-free (BSA/glycerol-free) OTUB2 mouse monoclonal antibody, clone OTI11B3 (formerly 11B3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB2 (RC209650, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB2. Positive lysates LY402958 (100ug) and LC402958 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OTUB2 monoclonal antibody.

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  HEK293T cells transfected with either RC209650 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OTUB2 antibody (TA501946), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI11B3
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human OTUB2 (NP_075601) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.0 kDa
• Gene Name: Homo sapiens OTU deubiquitinase, ubiquitin aldehyde binding 2 (OTUB2), mRNA.
• Database Link:
  NP_075601
  Entrez Gene 68149 MouseEntrez Gene 314405 RatEntrez Gene 480420 DogEntrez Gene 706795 MonkeyEntrez Gene 78990 Human
  UniProt ID Q96DC9
• Background: Otubains are deubiquitylating cysteine proteases (DUBs; see MIM 602519) that belong to the ovarian tumor (OTU) protein superfamily. Like other DUBs, otubains cleave proteins precisely at the ubiquitin (UB; see MIM 191339)-protein bond. [supplied by OMIM]
• Synonyms: C14orf137; OTB2; OTU2

Reference Data

• Protein Families: Protease