SAMHD1 Mouse Monoclonal Antibody [Clone ID: OTI1F6]

CAT#: CF501953

Carrier-free (BSA/glycerol-free) SAMHD1 mouse monoclonal antibody, clone OTI1F6 (formerly 1F6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SAMHD1 monoclonal antibody (1:200).

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SAMHD1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Anti-SAMHD1 mouse monoclonal antibody (TA501953) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).

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  Figure from citation: Immunofluorescence of SAMHD1 protein level by using anti-SAMHD1 antibody in human CD4+ T cells. View Citation

• HEK293T cells transfected with either RC206013 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAMHD1 antibody (TA501953), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA501953), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1F6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAMHD1 (NP_056289) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.0 kDa
• Gene Name: Homo sapiens SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1 (SAMHD1), transcript variant 1, mRNA.
• Database Link:
  NP_056289
  Entrez Gene 709060 MonkeyEntrez Gene 25939 Human
  UniProt ID Q9Y3Z3
• Background: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: CHBL2; DCIP; HDDC1; MOP-5; SBBI88