HAO1 Mouse Monoclonal Antibody [Clone ID: OTI5F4]

CAT#: CF501957

Carrier-free (BSA/glycerol-free) HAO1 mouse monoclonal antibody, clone OTI5F4 (formerly 5F4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAO1 (RC216834, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAO1. Positive lysates LY413692 (100ug) and LC413692 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC216834 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HAO1 antibody (TA501957), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-HAO1 antibody (TA501957), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-HAO1 antibody (TA501957), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI5F4
• Applications: FC, WB
• Recommended Dilution: WB 1:1000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HAO1 (NP_060015) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40.7 kDa
• Gene Name: Homo sapiens hydroxyacid oxidase 1 (HAO1), mRNA.
• Database Link:
  NP_060015
  Entrez Gene 15112 MouseEntrez Gene 311446 RatEntrez Gene 54363 Human
  UniProt ID Q9UJM8
• Background: This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq]. COMPLETENESS: full length.
• Synonyms: GOX; GOX1; HAOX1

Reference Data

• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways