SPR Mouse Monoclonal Antibody [Clone ID: OTI3A9]

CAT#: CF501961

Carrier-free (BSA/glycerol-free) SPR mouse monoclonal antibody, clone OTI3A9 (formerly 3A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPR (RC205679, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPR. Positive lysates LY401086 (100ug) and LC401086 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SPR monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and SPR-Knockout Hela cells (KO, Cat# LC810223) were separated by SDS-PAGE and immunoblotted with anti-SPR monoclonal antibody TA501961. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).

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  Anti-SPR mouse monoclonal antibody (TA501961) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SPR (RC205679).

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  HEK293T cells transfected with either RC205679 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SPR antibody (TA501961), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI3A9
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SPR (NP_003115) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.9 kDa
• Gene Name: Homo sapiens sepiapterin reductase (SPR), mRNA.
• Database Link:
  NP_003115
  Entrez Gene 705317 MonkeyEntrez Gene 6697 Human
  UniProt ID P35270
• Background: This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1. [provided by RefSeq]
• Synonyms: SDR38C1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Folate biosynthesis, Metabolic pathways