BIN3 Mouse Monoclonal Antibody [Clone ID: OTI5E3]

CAT#: CF501966

Carrier-free (BSA/glycerol-free) BIN3 mouse monoclonal antibody, clone OTI5E3 (formerly 5E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIN3 (RC203378, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIN3. Positive lysates LY402705 (100ug) and LC402705 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BIN3 monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BIN3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501966)

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  Anti-BIN3 mouse monoclonal antibody (TA501966) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BIN3 (RC203378).

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  HEK293T cells transfected with either RC203378 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BIN3 antibody (TA501966), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI5E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BIN3 (NP_061158) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 29.5 kDa
• Gene Name: Homo sapiens bridging integrator 3 (BIN3), transcript variant 1, mRNA.
• Database Link:
  NP_061158
  Entrez Gene 57784 MouseEntrez Gene 361065 RatEntrez Gene 608969 DogEntrez Gene 708840 MonkeyEntrez Gene 55909 Human
  UniProt ID Q9NQY0
• Background: The product of this gene is a member of the BAR domain protein family. The encoded protein is comprised solely of a BAR domain which is predicted to form coiled-coil structures and proposed to mediate dimerization, sense and induce membrane curvature, and bind small GTPases. BAR domain proteins have been implicated in endocytosis, intracellular transport, and a diverse set of other processes. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: MGC14978