Bestrophin 3 (BEST3) Mouse Monoclonal Antibody [Clone ID: OTI2H3]

CAT#: CF501979

Carrier-free (BSA/glycerol-free) BEST3 mouse monoclonal antibody, clone OTI2H3 (formerly 2H3)

Product Images

Anti-BEST3 mouse monoclonal antibody (TA501979) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BEST3 (RC218436).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BEST3 (RC218436, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BEST3. Positive lysates LY407540 (100ug) and LC407540 (20ug) can be purchased separately from OriGene.

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  Flow cytometric Analysis of Hela cells, using anti-BEST3 antibody (TA501979), (Red), compared to a nonspecific negative control antibody, (Blue).

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  HEK293T cells transfected with either RC218436 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BEST3 antibody (TA501979), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2H3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BEST3 (NP_689652) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 50.8 kDa
• Gene Name: Homo sapiens bestrophin 3 (BEST3), transcript variant 2, mRNA.
• Database Link:
  NP_689652
  Entrez Gene 144453 Human
  UniProt ID Q8N1M1
• Background: BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]). [supplied by OMIM]
• Synonyms: VMD2L3

Reference Data

• Protein Families: Ion Channels: Other, Transmembrane