HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5]

CAT#: CF502030

Carrier-free (BSA/glycerol-free) HDAC6 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (RC209649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6. Positive lysates LY401822 (100ug) and LC401822 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.

• 

  Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

• 

  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

• 

  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC6 mouse monoclonal antibody (TA502030) at 1:150 dilution.

• Anti-HDAC6 mouse monoclonal antibody (TA502030) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6 (RC209649).

• HEK293T cells transfected with either RC209649 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody (TA502030), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-HDAC6 antibody (TA502030), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-HDAC6 antibody (TA502030), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI4C5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100, FLOW 1:100, IHC 1:150
• Reactivities: Human, Monkey, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 131.2 kDa
• Gene Name: Homo sapiens histone deacetylase 6 (HDAC6), transcript variant 5, mRNA.
• Database Link:
  NP_006035
  Entrez Gene 84581 RatEntrez Gene 480907 DogEntrez Gene 711363 MonkeyEntrez Gene 10013 Human
  UniProt ID Q9UBN7
• Background: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription. [provided by RefSeq]
• Synonyms: CPBHM; HD6; JM21; PPP1R90

Reference Data

• Protein Families: Druggable Genome, Transcription Factors