Annexin A3 (ANXA3) Mouse Monoclonal Antibody [Clone ID: OTI1A9]

CAT#: CF502048

Carrier-free (BSA/glycerol-free) ANXA3 mouse monoclonal antibody, clone OTI1A9 (formerly 1A9)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ANXA3 (RC201540, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ANXA3. Positive lysates LY417500 (100ug) and LC417500 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ANXA3 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and ANXA3-Knockout HeLa cells (KO, Cat# LC812280) were separated by SDS-PAGE and immunoblotted with anti-ANXA3 monoclonal antibody TA502048 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  HEK293T cells transfected with either RC201540 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ANXA3 antibody (TA502048), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1A9
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ANXA3 (NP_005130) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.2 kDa
• Gene Name: Homo sapiens annexin A3 (ANXA3), mRNA.
• Database Link:
  NP_005130
  Entrez Gene 11745 MouseEntrez Gene 25291 RatEntrez Gene 478447 DogEntrez Gene 697239 MonkeyEntrez Gene 306 Human
  UniProt ID P12429
• Background: This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq]
• Synonyms: ANX3

Reference Data

• Protein Families: Stem cell - Pluripotency