OSBPL11 Mouse Monoclonal Antibody [Clone ID: OTI6A6]

CAT#: CF502068

Carrier-free (BSA/glycerol-free) OSBPL11 mouse monoclonal antibody, clone OTI6A6 (formerly 6A6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OSBPL11 (RC209418, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OSBPL11. Positive lysates LY411565 (100ug) and LC411565 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-OSBPL11 monoclonal antibody.

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  Anti-OSBPL11 mouse monoclonal antibody (TA502068) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OSBPL11 (RC209418).

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  Flow cytometric Analysis of Hela cells, using anti-OSBPL11 antibody (TA502068), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-OSBPL11 antibody (TA502068), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI6A6
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human OSBPL11 (NP_073613) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 83.5 kDa
• Gene Name: Homo sapiens oxysterol binding protein like 11 (OSBPL11), mRNA.
• Database Link:
  NP_073613
  Entrez Gene 106326 MouseEntrez Gene 303888 RatEntrez Gene 114885 Human
  UniProt ID Q9BXB4
• Background: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: ORP-11; ORP11; OSBP12; TCCCIA00292