RASD2 Mouse Monoclonal Antibody [Clone ID: OTI1F7]

CAT#: CF502072

Carrier-free (BSA/glycerol-free) RASD2 mouse monoclonal antibody, clone OTI1F7 (formerly 1F7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RASD2 (RC201454, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RASD2. Positive lysates LY415366 (100ug) and LC415366 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RASD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502072)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RASD2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502072)

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  Anti-RASD2 mouse monoclonal antibody (TA502072) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RASD2 (RC201454).

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  HEK293T cells transfected with either RC201454 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RASD2 antibody (TA502072), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1F7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RASD2 (NP_055125) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.2 kDa
• Gene Name: Homo sapiens RASD family member 2 (RASD2), transcript variant 1, mRNA.
• Database Link:
  NP_055125
  Entrez Gene 75141 MouseEntrez Gene 171099 RatEntrez Gene 23551 Human
  UniProt ID Q96D21
• Background: This gene encodes a Ras-related protein that enriched in striatum. The product of this gene binds to GTP and possesses intrinsic GTPase activity. The gene belongs to the Ras superfamily of small GTPases. The exact function of this gene is unknown, but most striatum-specific mRNAs characterized to date encode components of signal transduction cascades. [provided by RefSeq]
• Synonyms: MGC:4834; Rhes; TEM2

Reference Data

• Protein Families: Druggable Genome