CLPP Mouse Monoclonal Antibody [Clone ID: OTI1D3]

CAT#: CF502075

Carrier-free (BSA/glycerol-free) CLPP mouse monoclonal antibody, clone OTI1D3 (formerly 1D3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLPP (RC200301, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CLPP. Positive lysates LY416925 (100ug) and LC416925 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CLPP monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and CLPP-Knockout 293T cells (KO, Cat# LC811671) were separated by SDS-PAGE and immunoblotted with anti-CLPP monoclonal antibody TA502075, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Anti-CLPP mouse monoclonal antibody (TA502075) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CLPP (RC200301).

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  HEK293T cells transfected with either RC200301 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CLPP antibody (TA502075), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CLPP (NP_006003) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 24.1 kDa
• Gene Name: Homo sapiens caseinolytic mitochondrial matrix peptidase proteolytic subunit (CLPP), mRNA.
• Database Link:
  NP_006003
  Entrez Gene 301117 RatEntrez Gene 611738 DogEntrez Gene 699593 MonkeyEntrez Gene 8192 Human
  UniProt ID Q16740
• Background: The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: DFNB81; PRLTS3

Reference Data

• Protein Families: Druggable Genome, Protease