GRHPR Mouse Monoclonal Antibody [Clone ID: OTI2H4]

CAT#: CF502091

Carrier-free (BSA/glycerol-free) GRHPR mouse monoclonal antibody, clone OTI2H4 (formerly 2H4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRHPR (RC200963, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRHPR. Positive lysates LY415912 (100ug) and LC415912 (20ug) can be purchased separately from OriGene.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and GRHPR-Knockout 293T cells (KO, Cat# LC841943) were separated by SDS-PAGE and immunoblotted with anti-GRHPR monoclonal antibody TA502091 (1:500`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GRHPR mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502091)

• HEK293T cells transfected with either RC200963 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRHPR antibody (TA502091), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2H4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GRHPR (NP_036335) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.5 kDa
• Gene Name: Homo sapiens glyoxylate and hydroxypyruvate reductase (GRHPR), mRNA.
• Database Link:
  NP_036335
  Entrez Gene 680021 RatEntrez Gene 9380 Human
  UniProt ID Q9UBQ7
• Background: This gene encodes an enzyme with hydroxypyruvate reductase, glyoxylate reductase, and D-glycerate dehydrogenase enzymatic activities. The enzyme has widespread tissue expression and has a role in metabolism. Type II hyperoxaluria is caused by mutations in this gene. [provided by RefSeq, Jul 2008]
• Synonyms: GLXR; GLYD; PH2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism