CALCOCO2 Mouse Monoclonal Antibody [Clone ID: OTI7A7]

CAT#: CF502106

Carrier-free (BSA/glycerol-free) CALCOCO2 mouse monoclonal antibody, clone OTI7A7 (formerly 7A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CALCOCO2 (RC203843, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CALCOCO2 (1:2000).

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  Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY CALCOCO2 (RC203843) using anti-CALCOCO2 antibody (TA502106/green, upper left; DAPI/blue, lower left; MERGED, upper right). 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).

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  HEK293T cells transfected with either RC203843 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CALCOCO2 antibody (TA502106), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI7A7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CALCOCO2 (NP_005822) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 52.1 kDa
• Gene Name: Homo sapiens calcium binding and coiled-coil domain 2 (CALCOCO2), transcript variant 3, mRNA.
• Database Link:
  NP_005822
  Entrez Gene 10241 Human
  UniProt ID Q13137
• Background: The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]. COMPLETENESS: complete on the 3' end.
• Synonyms: NDP52