LMAN1 Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CAT#: CF502138

Carrier-free (BSA/glycerol-free) LMAN1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LMAN1 (RC207088, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LMAN1. Positive lysates LY401709 (100ug) and LC401709 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LMAN1 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and LMAN1-Knockout 293T cells (KO, Cat# LC812175) were separated by SDS-PAGE and immunoblotted with anti-LMAN1 monoclonal antibody TA502138, (1:1000). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-LMAN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502138)

• Anti-LMAN1 mouse monoclonal antibody (TA502138) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LMAN1 (RC207088).

• HEK293T cells transfected with either RC207088 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LMAN1 antibody (TA502138), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-LMAN1 antibody (TA502138), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1E3
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human LMAN1 (NP_005561) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.2 kDa
• Gene Name: Homo sapiens lectin, mannose binding 1 (LMAN1), mRNA.
• Database Link:
  NP_005561
  Entrez Gene 70361 MouseEntrez Gene 116666 RatEntrez Gene 476186 DogEntrez Gene 3998 Human
  UniProt ID P49257
• Background: The protein encoded by this gene is a type I integral membrane protein localized in the intermediate region between the endoplasmic reticulum and the Golgi, presumably recycling between the two compartments. The protein is a mannose-specific lectin and is a member of a novel family of plant lectin homologs in the secretory pathway of animal cells. Mutations in the gene are associated with a coagulation defect. Using positional cloning, the gene was identified as the disease gene leading to combined factor V-factor VIII deficiency, a rare, autosomal recessive disorder in which both coagulation factors V and VIII are diminished. [provided by RefSeq]
• Synonyms: ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; MCFD1; MR60

Reference Data

• Protein Families: Druggable Genome, Transmembrane