GMDS Mouse Monoclonal Antibody [Clone ID: OTI2A1]

CAT#: CF502141

Carrier-free (BSA/glycerol-free) GMDS mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GMDS (RC200471, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GMDS. Positive lysates LY419903 (100ug) and LC419903 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-GMDS monoclonal antibody (1:200).

Specifications

Product Data

• Clone Name: OTI2A1
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GMDS (NP_001491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.8 kDa
• Gene Name: Homo sapiens GDP-mannose 4,6-dehydratase (GMDS), transcript variant 1, mRNA.
• Database Link:
  NP_001491
  Entrez Gene 2762 Human
  UniProt ID O60547
• Background: GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020). [supplied by OMIM]
• Synonyms: GMD; SDR3E1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Metabolic pathways