HDAC1 Mouse Monoclonal Antibody [Clone ID: OTI5C3]

CAT#: CF502142

Carrier-free (BSA/glycerol-free) HDAC1 mouse monoclonal antibody, clone OTI5C3 (formerly 5C3)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC1 (RC201745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC1. Positive lysates LY417624 (100ug) and LC417624 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC1 monoclonal antibody.

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  Anti-HDAC1 mouse monoclonal antibody (TA502142) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC1 (RC201745).

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  HEK293T cells transfected with either RC201745 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC1 antibody (TA502142), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5C3
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:200~500, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HDAC1 (NP_004955) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54.9 kDa
• Gene Name: Homo sapiens histone deacetylase 1 (HDAC1), mRNA.
• Database Link:
  NP_004955
  Entrez Gene 297893 RatEntrez Gene 433759 MouseEntrez Gene 708441 MonkeyEntrez Gene 3065 Human
  UniProt ID Q13547
• Background: Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq]
• Synonyms: GON-10; HD1; RPD3; RPD3L1

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Transcription Factors
• Protein Pathways: Cell cycle, Chronic myeloid leukemia, Huntington's disease, Notch signaling pathway, Pathways in cancer