SAMHD1 Mouse Monoclonal Antibody [Clone ID: OTI1A1]

CAT#: CF502157

Carrier-free (BSA/glycerol-free) SAMHD1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)

Product Images

Figure A, Western blot analysis of overexpressed lysates(25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, lane 1) , human SAMHD1 plasmid (RC206013, lane 2) using anti-SAMHD1 antibody TA502157 (1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (10ug) from a mouse tissue by using anti-SAMHD1 monoclonal antibody (1:200).

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  Western blot analysis of extracts (50ug per lane) from 4 cell lines lysates by using anti-VHL monoclonal antibody(TA502157, 1:500)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SAMHD1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Anti-SAMHD1 mouse monoclonal antibody (TA502157) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).

• HEK293T cells transfected with either RC206013 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SAMHD1 antibody (TA502157), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA502157), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody (TA502157), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1A1
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SAMHD1(NP_056289) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72 kDa
• Gene Name: SAM and HD domain containing deoxynucleoside triphosphate triphosphohydrolase 1
• Database Link:
  NP_056289
  Entrez Gene 56045 MouseEntrez Gene 311580 RatEntrez Gene 709060 MonkeyEntrez Gene 25939 Human
  UniProt ID Q9Y3Z3
• Background: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq]
• Synonyms: CHBL2; DCIP; HDDC1; MOP-5; SBBI88