CDCP1 Mouse Monoclonal Antibody [Clone ID: OTI2B8]

CAT#: CF502171

Carrier-free (BSA/glycerol-free) CDCP1 mouse monoclonal antibody, clone OTI2B8 (formerly 2B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDCP1 (RC220633, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDCP1. Positive lysates LY402953 (100ug) and LC402953 (20ug) can be purchased separately from OriGene.

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  Western Blot analysis of extracts (35ug) from 5 different cell lines by using anti-CDCP1 monoclonal antibody (Clone 2B8)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-CDCP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502171)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-CDCP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502171)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-CDCP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502171)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-CDCP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502171)

• Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-CDCP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502171)

• Flow cytometric Analysis of Jurkat cells, using anti-CDCP1 antibody (TA502171), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2B8
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CDCP1 (NP_073753) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 92.8 kDa
• Gene Name: Homo sapiens CUB domain containing protein 1 (CDCP1), transcript variant 1, mRNA.
• Database Link:
  NP_073753
  Entrez Gene 64866 Human
  UniProt ID Q9H5V8
• Background: The protein encoded by this gene is a transmembrane protein containing three extracellular CUB domains. This protein is found to be overexpressed in colon and lung cancers. Its expression level is correlated with the metastatic ability of carcinoma cells. This protein is located on the cell surface. It has been shown to be tyrosine phosphorylated in a cancer cell line. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
• Synonyms: CD318; SIMA135; TRASK

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane