MSI1 Mouse Monoclonal Antibody [Clone ID: OTI1G8]

CAT#: CF502198

Carrier-free (BSA/glycerol-free) MSI1 mouse monoclonal antibody, clone OTI1G8 (formerly 1G8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MSI1 (RC215992, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MSI1. Positive lysates LY419331 (100ug) and LC419331 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MSI1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502198)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502198)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-MSI1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502198)

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  Anti-MSI1 mouse monoclonal antibody (TA502198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MSI1 (RC215992).

• HEK293T cells transfected with either RC215992 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MSI1 antibody (TA502198), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-MSI1 antibody (TA502198), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI1G8
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MSI1 (NP_002433) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.9 kDa
• Gene Name: Homo sapiens musashi RNA binding protein 1 (MSI1), mRNA.
• Database Link:
  NP_002433
  Entrez Gene 17690 MouseEntrez Gene 259272 RatEntrez Gene 699286 MonkeyEntrez Gene 4440 Human
  UniProt ID O43347
• Background: This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]
• Synonyms: musashi 1; musashi homolog 1 (Drosophila)