PACSIN3 Mouse Monoclonal Antibody [Clone ID: OTI4G7]

CAT#: CF502214

Carrier-free (BSA/glycerol-free) PACSIN3 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PACSIN3 (RC204103, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3. Positive lysates LY414084 (100ug) and LC414084 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PACSIN3 monoclonal antibody.

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  Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PACSIN3-Knockout 293T cells (KO, Cat# LC812043) were separated by SDS-PAGE and immunoblotted with anti-PACSIN3 monoclonal antibody TA502214, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.

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  HEK293T cells transfected with either RC204103 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PACSIN3 antibody (TA502214), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI4G7
• Applications: FC, WB
• Recommended Dilution: WB 1:200~500, FLOW 1:100
• Reactivities: Human, Monkey, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PACSIN3 (NP_057307) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48.3 kDa
• Gene Name: Homo sapiens protein kinase C and casein kinase substrate in neurons 3 (PACSIN3), transcript variant 2, mRNA.
• Database Link:
  NP_057307
  Entrez Gene 80708 MouseEntrez Gene 311187 RatEntrez Gene 475984 DogEntrez Gene 713919 MonkeyEntrez Gene 29763 Human
  UniProt ID Q9UKS6
• Background: This gene is a member of the protein kinase C and casein kinase substrate in neurons family. The encoded protein is involved in linking the actin cytoskeleton with vesicle formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: SDPIII

Reference Data

• Protein Families: Druggable Genome