PPM1G Mouse Monoclonal Antibody [Clone ID: OTI1C12]

CAT#: CF502232

Carrier-free (BSA/glycerol-free) PPM1G mouse monoclonal antibody, clone OTI1C12 (formerly 1C12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1G (RC213685, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1G. Positive lysates LY406071 (100ug) and LC406071 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1G monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Anti-PPM1G mouse monoclonal antibody (TA502232) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPM1G (RC213685).

Specifications

Product Data

• Clone Name: OTI1C12
• Applications: IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PPM1G (NP_817092) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.1 kDa
• Gene Name: Homo sapiens protein phosphatase, Mg2+/Mn2+ dependent 1G (PPM1G), mRNA.
• Database Link:
  NP_817092
  Entrez Gene 475703 DogEntrez Gene 5496 Human
  UniProt ID O15355
• Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]
• Synonyms: PP2CG; PP2CGAMMA; PPP2CG

Reference Data

• Protein Families: Druggable Genome, Phosphatase