BCL10 Mouse Monoclonal Antibody [Clone ID: OTI6A4]

CAT#: CF502233

Carrier-free (BSA/glycerol-free) BCL10 mouse monoclonal antibody, clone OTI6A4 (formerly 6A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCL10 (RC208752, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCL10. Positive lysates LY418351 (100ug) and LC418351 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-BCL10 monoclonal antibody (1:200).

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  Anti-BCL10 mouse monoclonal antibody (TA502233) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BCL10 (RC208752).

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  HEK293T cells transfected with either RC208752 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCL10 antibody (TA502233), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI6A4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCL10 (NP_003912) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 26.1 kDa
• Gene Name: Homo sapiens BCL10 immune signaling adaptor (BCL10), transcript variant 1, mRNA.
• Database Link:
  NP_003912
  Entrez Gene 12042 MouseEntrez Gene 83477 RatEntrez Gene 8915 Human
  UniProt ID O95999
• Background: This gene was identified by its translocation in a case of mucosa-associated lymphoid tissue (MALT) lymphoma. The protein encoded by this gene contains a caspase recruitment domain (CARD), and has been shown to induce apoptosis and to activate NF-kappaB. This protein is reported to interact with other CARD domain containing proteins including CARD9, 10, 11 and 14, which are thought to function as upstream regulators in NF-kappaB signaling. This protein is found to form a complex with MALT1, a protein encoded by another gene known to be translocated in MALT lymphoma. MALT1 and this protein are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy. [provided by RefSeq]
• Synonyms: c-E10; CARMEN; CIPER; CLAP; IMD37; mE10

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: B cell receptor signaling pathway, T cell receptor signaling pathway