Butyrylcholinesterase (BCHE) Mouse Monoclonal Antibody [Clone ID: OTI1E1]

CAT#: CF502307

Carrier-free (BSA/glycerol-free) BCHE mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BCHE (RC202198, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BCHE. Positive lysates LY424950 (100ug) and LC424950 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BCHE monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  HEK293T cells transfected with either RC202198 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BCHE antibody (TA502307), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1E1
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human BCHE (NP_000046) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.2 kDa
• Gene Name: Homo sapiens butyrylcholinesterase (BCHE), transcript variant 1, mRNA.
• Database Link:
  NP_000046
  Entrez Gene 488143 DogEntrez Gene 590 Human
  UniProt ID P06276
• Background: Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq, Jul 2008]
• Synonyms: CHE1; CHE2; E1

Reference Data

• Protein Families: Druggable Genome, Transmembrane