FAM40A (STRIP1) Mouse Monoclonal Antibody [Clone ID: OTI7G7]

CAT#: CF502314

Carrier-free (BSA/glycerol-free) FAM40A mouse monoclonal antibody, clone OTI7G7 (formerly 7G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FAM40A (RC210537, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FAM40A. Positive lysates LY409738 (100ug) and LC409738 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FAM40A monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502314)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502314)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502314)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-FAM40A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502314)

• Anti-FAM40A mouse monoclonal antibody (TA502314) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FAM40A (RC210537).

• HEK293T cells transfected with either RC210537 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FAM40A antibody (TA502314), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI7G7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human FAM40A (NP_149079) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 95.4 kDa
• Gene Name: Homo sapiens striatin interacting protein 1 (STRIP1), transcript variant 1, mRNA.
• Database Link:
  NP_149079
  Entrez Gene 362012 RatEntrez Gene 490117 DogEntrez Gene 85369 Human
  UniProt ID Q5VSL9
• Synonyms: FAM40A; FAR11A