DNAJB2 Mouse Monoclonal Antibody [Clone ID: OTI5C11]

CAT#: CF502329

Carrier-free (BSA/glycerol-free) DNAJB2 mouse monoclonal antibody, clone OTI5C11 (formerly 5C11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB2 (RC206769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB2. Positive lysates LY422070 (100ug) and LC422070 (20ug) can be purchased separately from OriGene.

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  HEK293T cells transfected with either RC206769 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DNAJB2 antibody (TA502329), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5C11
• Applications: FC, WB
• Recommended Dilution: WB 1:2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DNAJB2 (NP_001034639) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 30.4 kDa
• Gene Name: Homo sapiens DnaJ heat shock protein family (Hsp40) member B2 (DNAJB2), transcript variant 1, mRNA.
• Database Link:
  NP_001034639
  Entrez Gene 3300 Human
  UniProt ID P25686
• Background: This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
• Synonyms: CMT2T; DSMA5; HSJ-1; HSJ1; HSPF3