DCLRE1B Mouse Monoclonal Antibody [Clone ID: OTI2B7]

CAT#: CF502352

Carrier-free (BSA/glycerol-free) DCLRE1B mouse monoclonal antibody, clone OTI2B7 (formerly 2B7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DCLRE1B (RC205744, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DCLRE1B. Positive lysates LY411532 (100ug) and LC411532 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-DCLRE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502352)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DCLRE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502352)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-DCLRE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502352)

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  Anti-DCLRE1B mouse monoclonal antibody (TA502352) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DCLRE1B (RC205744) (1:100).

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  HEK293T cells transfected with either RC205744 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DCLRE1B antibody (TA502352), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-DCLRE1B antibody (TA502352), (Red), compared to a nonspecific negative control antibody, (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DCLRE1B antibody (TA502352), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI2B7
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:200~500, IHC 1:150, FLOW 1:100
• Reactivities: Human, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-280 of human DCLRE1B(NP_073747) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 59.8 kDa
• Gene Name: Homo sapiens DNA cross-link repair 1B (DCLRE1B), transcript variant 1, mRNA.
• Database Link:
  NP_073747
  Entrez Gene 64858 Human
  UniProt ID Q9H816
• Background: DNA interstrand cross-links prevent strand separation, thereby physically blocking transcription, replication, and segregation of DNA. DCLRE1B is one of several evolutionarily conserved genes involved in repair of interstrand cross-links (Dronkert et al., 2000 [PubMed 10848582]). [supplied by OMIM]
• Synonyms: APOLLO; SNM1B; SNMIB

Reference Data

• Protein Families: Druggable Genome