Tristetraprolin (ZFP36) Mouse Monoclonal Antibody [Clone ID: OTI4B10]

CAT#: CF502378

Carrier-free (BSA/glycerol-free) ZFP36 mouse monoclonal antibody, clone OTI4B10 (formerly 4B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP36 (RC202049, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP36. Positive lysates LY401161 (100ug) and LC401161 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ZFP36 monoclonal antibody.

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  HEK293T cells transfected with either RC202049 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ZFP36 antibody (TA502378), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-ZFP36 antibody (TA502378), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-ZFP36 antibody (TA502378), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4B10
• Applications: FC, WB
• Recommended Dilution: WB 1:500~2000, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ZFP36(NP_003398) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.8 kDa
• Gene Name: Homo sapiens ZFP36 ring finger protein (ZFP36), mRNA.
• Database Link:
  NP_003398
  Entrez Gene 7538 Human
  UniProt ID P26651
• Synonyms: G0S24; GOS24; NUP475; RNF162A; TIS11; TTP; zfp-36