PDE2A Mouse Monoclonal Antibody [Clone ID: OTI3A4]

CAT#: CF502389

Carrier-free (BSA/glycerol-free) PDE2A mouse monoclonal antibody, clone OTI3A4 (formerly 3A4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A. Positive lysates LY419215 (100ug) and LC419215 (20ug) can be purchased separately from OriGene.

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502389)

• 

  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PDE2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502389)

• 

  Anti-PDE2A mouse monoclonal antibody (TA502389) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A (RC207219).

• 

  HEK293T cells transfected with either RC207219 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE2A antibody (TA502389), and then analyzed by flow cytometry.

• 

  Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody (TA502389), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 6 images

Specifications

Product Data

• Clone Name: OTI3A4
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PDE2A (NP_002590) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 105.5 kDa
• Gene Name: Homo sapiens phosphodiesterase 2A (PDE2A), transcript variant 1, mRNA.
• Database Link:
  NP_002590
  Entrez Gene 81743 RatEntrez Gene 207728 MouseEntrez Gene 5138 Human
  UniProt ID O00408
• Synonyms: CGS-PDE; cGSPDE; PDE2A1; PED2A4

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism