VCAM1 Mouse Monoclonal Antibody [Clone ID: OTI4A10]

CAT#: CF502390

Carrier-free (BSA/glycerol-free) VCAM1 mouse monoclonal antibody, clone OTI4A10 (formerly 4A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VCAM1 (RC209761, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VCAM1. Positive lysates LY421491 (100ug) and LC421491 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VCAM1 monoclonal antibody.

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  Flow cytometric Analysis of Hela cells, using anti-VCAM1 antibody (TA502390), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Specifications

Product Data

• Clone Name: OTI4A10
• Applications: FC, WB
• Recommended Dilution: WB 1:200~500, FLOW 1:100
• Reactivities: Human, Monkey, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VCAM1 (NP_001069) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 78.7 kDa
• Gene Name: Homo sapiens vascular cell adhesion molecule 1 (VCAM1), transcript variant 1, mRNA.
• Database Link:
  NP_001069
  Entrez Gene 403982 DogEntrez Gene 712036 MonkeyEntrez Gene 7412 Human
  UniProt ID P19320
• Background: This gene is a member of the Ig superfamily and encodes a cell surface sialoglycoprotein expressed by cytokine-activated endothelium. This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. Two alternatively spliced transcripts encoding different isoforms have been described for this gene. [provided by RefSeq]
• Synonyms: CD106; INCAM-100

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration