Arginase 1 (ARG1) Mouse Monoclonal Antibody [Clone ID: OTI4E6]

CAT#: CF502413

Carrier-free (BSA/glycerol-free) ARG1 mouse monoclonal antibody, clone OTI4E6 (formerly 4E6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARG1 (Cat# RC204649, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARG1(Cat# TA502413). Positive lysates LY424951 (100ug) and LC424951 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARG1 monoclonal antibody.

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  Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ARG1 monoclonal antibody (1:200).

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  Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human ARG2 plasmid (RC206756, hARG2), human ARG1 plasmid (RC204649, hARG1) using anti-ARG1 antibody TA502413(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARG1 mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  HEK293T cells transfected with either RC204649 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARG1 antibody (TA502413), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4E6
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000~2000, IHC 1:50, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ARG1 (NP_000036) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34.6 kDa
• Gene Name: arginase 1
• Database Link:
  NP_000036
  Entrez Gene 11846 MouseEntrez Gene 29221 RatEntrez Gene 383 Human
  UniProt ID P05089
• Background: Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]
• Synonyms: arginase; arginase 1; liver; liver-type arginase; OTTHUMP00000017209; type I arginase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways