RTF2 Mouse Monoclonal Antibody [Clone ID: OTI7E10]

CAT#: CF502421

Carrier-free (BSA/glycerol-free) C20orf43 mouse monoclonal antibody, clone OTI7E10 (formerly 7E10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C20orf43 (RC201652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C20orf43. Positive lysates LY414003 (100ug) and LC414003 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-C20orf43 monoclonal antibody.

• 

  Anti-C20orf43 mouse monoclonal antibody (TA502421) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY C20orf43 (RC201652).

Specifications

Product Data

• Clone Name: OTI7E10
• Applications: IF, WB
• Recommended Dilution: WB 1:1000~2000, IF 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human C20orf43 (NP_057491) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.7 kDa
• Gene Name: Homo sapiens replication termination factor 2 (RTF2), transcript variant 2, mRNA.
• Database Link:
  NP_057491
  Entrez Gene 51507 Human
  UniProt ID Q9BY42
• Synonyms: C20orf43; CDAO5; HSPC164; SHUJUN-3