Beta TRCP (BTRC) Mouse Monoclonal Antibody [Clone ID: OTI2H2]

CAT#: CF502465

Carrier-free (BSA/glycerol-free) BTRC mouse monoclonal antibody, clone OTI2H2 (formerly 2H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY BTRC (Cat# RC207025, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTRC(Cat# TA502465).

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-BTRC monoclonal antibody at 1:200 dilution.

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  Western blot analysis of extracts (10ug) from a mouse cell line by using anti-BTRC monoclonal antibody (1:200).

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  Anti-BTRC mouse monoclonal antibody (TA502465) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BTRC (RC207025).

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  HEK293T cells transfected with either RC207025 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BTRC antibody (TA502465), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2H2
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:1000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 52-354 of human BTRC(NP_378663) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.7 kDa
• Gene Name: beta-transducin repeat containing E3 ubiquitin protein ligase
• Database Link:
  NP_378663
  Entrez Gene 12234 MouseEntrez Gene 361765 RatEntrez Gene 8945 Human
  UniProt ID Q9Y297
• Background: This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbws class; in addition to an F-box, this protein contains multiple WD-40 repeats. The encoded protein mediates degradation of CD4 via its interaction with HIV-1 Vpu. It has also been shown to ubiquitinate phosphorylated NFKBIA (nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha), targeting it for degradation and thus activating nuclear factor kappa-B. Alternatively spliced transcript variants have been described. A related pseudogene exists in chromosome 6. [provided by RefSeq, Mar
• Synonyms: BETA-TRCP; betaTrCP; bTrCP; bTrCP1; FBW1A; FBXW1; FBXW1A; FWD1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Hedgehog signaling pathway, Oocyte meiosis, Ubiquitin mediated proteolysis, Wnt signaling pathway