PIH1D2 Mouse Monoclonal Antibody [Clone ID: OTI4A10]

CAT#: CF502467

Carrier-free (BSA/glycerol-free) PIH1D2 mouse monoclonal antibody, clone OTI4A10 (formerly 4A10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIH1D2 (RC205039, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIH1D2. Positive lysates LY408526 (100ug) and LC408526 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PIH1D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502467)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-PIH1D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502467)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-PIH1D2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502467)

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  Flow cytometric Analysis of Hela cells, using anti-PIH1D2 antibody (TA502467), (Red), compared to a nonspecific negative control antibody, (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-PIH1D2 antibody (TA502467), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI4A10
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:1000, IHC 1:150, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-315 of human PIH1D2(NP_620144) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 35.8 kDa
• Gene Name: Homo sapiens PIH1 domain containing 2 (PIH1D2), transcript variant 1, mRNA.
• Database Link:
  NP_620144
  Entrez Gene 120379 Human
  UniProt ID Q8WWB5
• Synonyms: PIH1 domain containing 2