RDH14 Mouse Monoclonal Antibody [Clone ID: OTI1A7]

CAT#: CF502478

Carrier-free (BSA/glycerol-free) RDH14 mouse monoclonal antibody, clone OTI1A7 (formerly 1A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RDH14 (RC203411, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RDH14. Positive lysates LY412210 (100ug) and LC412210 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RDH14 monoclonal antibody.

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  HEK293T cells transfected with either RC203411 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RDH14 antibody (TA502478), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Hela cells, using anti-RDH14 antibody (TA502478), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Jurkat cells, using anti-RDH14 antibody (TA502478), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI1A7
• Applications: FC, WB
• Recommended Dilution: WB 1:200~500, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human RDH14 (NP_065956) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 36.7 kDa
• Gene Name: Homo sapiens retinol dehydrogenase 14 (RDH14), mRNA.
• Database Link:
  NP_065956
  Entrez Gene 500629 RatEntrez Gene 57665 Human
  UniProt ID Q9HBH5
• Synonyms: PAN2; SDR7C4

Reference Data

• Protein Families: Druggable Genome, Transmembrane