Prostaglandin dehydrogenase 1 (HPGD) Mouse Monoclonal Antibody [Clone ID: OTI2F5]

CAT#: CF502484

Carrier-free (BSA/glycerol-free) HPGD mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD. Positive lysates LY400305 (100ug) and LC400305 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-HPGD monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502484)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502484)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502484)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502484)

• HEK293T cells transfected with either RC204160 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HPGD antibody (TA502484), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI2F5
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human HPGD (NP_000851) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28.8 kDa
• Gene Name: Homo sapiens 15-hydroxyprostaglandin dehydrogenase (HPGD), transcript variant 1, mRNA.
• Database Link:
  NP_000851
  Entrez Gene 3248 Human
  UniProt ID P15428
• Background: This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
• Synonyms: 15-PGDH; PGDH; PGDH1; PHOAR1; SDR36C1

Reference Data

• Protein Families: Druggable Genome