MAGEB18 Mouse Monoclonal Antibody [Clone ID: OTI1F5]

CAT#: CF502493

Carrier-free (BSA/glycerol-free) MAGEB18 mouse monoclonal antibody, clone OTI1F5 (formerly 1F5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MAGEB18 (RC206329, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MAGEB18. Positive lysates LY406567 (100ug) and LC406567 (20ug) can be purchased separately from OriGene.

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  Anti-MAGEB18 mouse monoclonal antibody (TA502493) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MAGEB18 (RC206329).

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  HEK293T cells transfected with either RC206329 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MAGEB18 antibody (TA502493), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1F5
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MAGEB18 (NP_775970) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38.3 kDa
• Gene Name: Homo sapiens MAGE family member B18 (MAGEB18), mRNA.
• Database Link:
  NP_775970
  Entrez Gene 286514 Human
  UniProt ID Q96M61
• Synonyms: MGC33889