PON1 Mouse Monoclonal Antibody [Clone ID: OTI2D4]

CAT#: CF502528

Carrier-free (BSA/glycerol-free) PON1 mouse monoclonal antibody, clone OTI2D4 (formerly 2D4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PON1 (RC210356, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PON1. Positive lysates LY400156 (100ug) and LC400156 (20ug) can be purchased separately from OriGene.

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  Standard curve for ELISA analysis with PON1 recombinant protein (dilution range from 0.8ng/ml to 200ng/ml) using PON1 Capture Antibody (Cat# TA502610) at 5ug/ml and HRP conjugated PON1 Detection mAb (Cat# TA502528/TA502611) at 0.16ug/ml.

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  Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502528)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502528)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PON1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502528)

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  Anti-PON1 mouse monoclonal antibody (TA502528) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PON1 (RC210356).

• HEK293T cells transfected with either RC210356 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PON1 antibody (TA502528), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-PON1 antibody (TA502528), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-PON1 antibody (TA502528), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI2D4
• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human PON1 (NP_000437) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.6 kDa
• Gene Name: Homo sapiens paraoxonase 1 (PON1), mRNA.
• Database Link:
  NP_000437
  Entrez Gene 5444 Human
  UniProt ID P27169
• Background: The enzyme encoded by this gene is an arylesterase that mainly hydrolyzes paroxon to produce p-nitrophenol. Paroxon is an organophosphorus anticholinesterase compound that is produced in vivo by oxidation of the insecticide parathion. Polymorphisms in this gene are a risk factor in coronary artery disease. The gene is found in a cluster of three related paraoxonase genes at 7q21.3. [provided by RefSeq]
• Synonyms: ESA; MVCD5; PON

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Metabolic pathways