NDUFB9 Mouse Monoclonal Antibody [Clone ID: OTI4F8]

CAT#: CF502553

Carrier-free (BSA/glycerol-free) NDUFB9 mouse monoclonal antibody, clone OTI4F8 (formerly 4F8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB9 (RC200223, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB9. Positive lysates LY417578 (100ug) and LC417578 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NDUFB9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

• 

  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NDUFB9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA502553)

Specifications

Product Data

• Clone Name: OTI4F8
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:500
• Reactivities: Human, Mouse, Rat, Dog
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 3-179 of human NDUFB9 (NP_001893) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 21.7 kDa
• Gene Name: Homo sapiens NADH:ubiquinone oxidoreductase subunit B9 (NDUFB9), transcript variant 1, mRNA.
• Database Link:
  NP_004996
  Entrez Gene 66218 MouseEntrez Gene 299954 RatEntrez Gene 475094 DogEntrez Gene 4715 Human
  UniProt ID Q9Y6M9
• Synonyms: B22; CI-B22; LYRM3; UQOR22

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease