MEF2C Mouse Monoclonal Antibody [Clone ID: OTI1H5]

CAT#: CF502565

Carrier-free (BSA/glycerol-free) MEF2C mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEF2C (RC220584, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEF2C. Positive lysates LY419349 (100ug) and LC419349 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (10ug) from 1 cell line by using anti-MEF2C monoclonal antibody (1:200).

• 

  Immunohistochemical staining of paraffin-embedded Human Ovary tissue within the normal limits using anti-MEF2C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502565)

• 

  Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-MEF2C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502565)

• 

  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MEF2C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502565)

• 

  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MEF2C mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502565)

• Anti-MEF2C mouse monoclonal antibody (TA502565) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MEF2C (RC220584 ).

• HEK293T cells transfected with either RC220584 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEF2C antibody (TA502565), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Hela cells, using anti-MEF2C antibody (TA502565), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-MEF2C antibody (TA502565), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

Show all 10 images

Specifications

Product Data

• Clone Name: OTI1H5
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MEF2C (NP_002388) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 51 kDa
• Gene Name: Homo sapiens myocyte enhancer factor 2C (MEF2C), transcript variant 1, mRNA.
• Database Link:
  NP_002388
  Entrez Gene 17260 MouseEntrez Gene 499497 RatEntrez Gene 4208 Human
  UniProt ID Q06413
• Background: This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq]
• Synonyms: C5DELq14.3; DEL5q14.3

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: MAPK signaling pathway