VASP Mouse Monoclonal Antibody [Clone ID: OTI4D6]

CAT#: CF502647

Carrier-free (BSA/glycerol-free) VASP mouse monoclonal antibody, clone OTI4D6 (formerly 4D6)

Product Images

Anti-VASP mouse monoclonal antibody (TA502647) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY VASP (RC203544 ).

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  HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY VASP (Cat# RC203544, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-VASP(Cat# TA502647). Positive lysates LY401150 (100ug) and LC401150 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-VASP monoclonal antibody.

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-VASP mouse monoclonal antibody. Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

• HEK293T cells transfected with either RC203544 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-VASP antibody (TA502647), and then analyzed by flow cytometry.

• Flow cytometric Analysis of Jurkat cells, using anti-VASP antibody (TA502647), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Hela cells, using anti-VASP antibody (TA502647), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and VASP-Knockout HeLa cells (KO, Cat# LC832038) were separated by SDS-PAGE and immunoblotted with anti-VASP monoclonal antibody TA502647 (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.

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Specifications

Product Data

• Clone Name: OTI4D6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human VASP(NP_003361) produced in 293T Cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 39.6 kDa
• Gene Name: vasodilator stimulated phosphoprotein
• Database Link:
  NP_003361
  Entrez Gene 22323 MouseEntrez Gene 361517 RatEntrez Gene 7408 Human
  UniProt ID P50552
• Background: Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. [provided by RefSeq]
• Synonyms: vasodilator-stimulated phosphoprotein

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Fc gamma R-mediated phagocytosis, Focal adhesion, Leukocyte transendothelial migration