CHCHD5 Mouse Monoclonal Antibody [Clone ID: OTI9E11]

CAT#: CF502668

Carrier-free (BSA/glycerol-free) CHCHD5 mouse monoclonal antibody, clone OTI9E11 (formerly 9E11)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHCHD5 (RC202606, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHCHD5. Positive lysates LY410209 (100ug) and LC410209 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CHCHD5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502668)

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  HEK293T cells transfected with either RC202606 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CHCHD5 antibody (TA502668), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-CHCHD5 antibody (TA502668), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Flow cytometric Analysis of Hela cells, using anti-CHCHD5 antibody (TA502668), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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  Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-CHCHD5 monoclonal antibody (1:200).

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Specifications

Product Data

• Clone Name: OTI9E11
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CHCHD5 (NP_115685) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 12.2 kDa
• Gene Name: Homo sapiens coiled-coil-helix-coiled-coil-helix domain containing 5 (CHCHD5), transcript variant 1, mRNA.
• Database Link:
  NP_115685
  Entrez Gene 84269 Human
  UniProt ID Q9BSY4
• Synonyms: C2orf9; MIC14