DEF8 Mouse Monoclonal Antibody [Clone ID: OTI4B6]

CAT#: CF502669

Carrier-free (BSA/glycerol-free) DEF8 mouse monoclonal antibody, clone OTI4B6 (formerly 4B6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF8 (RC224669, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DEF8. Positive lysates LY403723 (100ug) and LC403723 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-DEF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502669)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-DEF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502669)

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-DEF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502669)

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  Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-DEF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502669)

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  Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-DEF8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA502669)

• Anti-DEF8 mouse monoclonal antibody (TA502669) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DEF8 (RC224669 ).

• Flow cytometric Analysis of Hela cells, using anti-DEF8 antibody (TA502669), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

• Flow cytometric Analysis of Jurkat cells, using anti-DEF8 antibody (TA502669), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).

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Specifications

Product Data

• Clone Name: OTI4B6
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human DEF8 (NP_997397) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 58.5 kDa
• Gene Name: Homo sapiens differentially expressed in FDCP 8 homolog (DEF8), transcript variant 1, mRNA.
• Database Link:
  NP_997397
  Entrez Gene 23854 MouseEntrez Gene 307973 RatEntrez Gene 54849 Human
  UniProt ID Q6ZN54
• Synonyms: FLJ20186; MGC104349