RNF156 (MGRN1) Mouse Monoclonal Antibody [Clone ID: OTI3A1]

CAT#: CF502681

Carrier-free (BSA/glycerol-free) MGRN1 mouse monoclonal antibody, clone OTI3A1 (formerly 3A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGRN1 (RC208284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGRN1. Positive lysates LY414714 (100ug) and LC414714 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MGRN1 monoclonal antibody.

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  Anti-MGRN1 mouse monoclonal antibody (TA502681) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MGRN1 (RC208284).

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  HEK293T cells transfected with either RC208284 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MGRN1 antibody (TA502681), and then analyzed by flow cytometry.

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  Flow cytometric Analysis of Jurkat cells, using anti-MGRN1 antibody (TA502681), (Red), compared to a nonspecific negative control antibody, (Blue).

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Specifications

Product Data

• Clone Name: OTI3A1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:500~2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MGRN1 (NP_056061) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 63 kDa
• Gene Name: Homo sapiens mahogunin ring finger 1 (MGRN1), transcript variant 1, mRNA.
• Database Link:
  NP_056061
  Entrez Gene 23295 Human
  UniProt ID O60291
• Background: Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro. [supplied by OMIM]
• Synonyms: RNF156

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis